The ChroLine software is by the time now one of the most efficiant software products for the purification of synthesis or natural products from mg to hectogramm scale under laboratory conditions. (Publication in LaborPraxis 9/2001).

Only small columns are needed since the ballistic chromatography mode allows hundreds of preparative runs with the same column. An intuitive user interface with intelligent auto-adaptive algorithms allows the onset of sophisticated parameters with a few mouse-drawn lines in a test chromatogram. By that, the parameter optimisation takes place during the first few repetitions, after that, the system can be left alone for hours and days.

The strictly reproducible "ballistic state" of the column is acheived by a short rinsing phase with appr. 1-2 column volumes of a strong eluent - and then in a fast step the immediate change of solvent composition towards the weakly eluting separation conditions. The sample is injected after one or two column volumes of the weak eluent.

Compared to analytical HPLC, the separation eluent is weaker for similar sample components. While the fractionnation of one sample portion still takes place, the next sample already is injected.

The injection is done by an HPLC-Pump working in volumetric mode.